Methicillin-resistant Staphylococcus aureus (MRSA), NAA

CPT: 87641
Updated on 08/21/2024
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Synonyms

  • MRSA NAA
  • MRSA PCR

Expected Turnaround Time

1 - 3 days



Related Documents

For more information, please view the literature below.

Microbiology Specimen Collection and Transport Guide


Specimen Requirements


Specimen

Nasal swab


Volume

Dual swab (preferred) or ESwab (preferred) or single swab

One swab

Dual swab (preferred) or ESwab (preferred) or single swab


Minimum Volume

Dual swab; 1 ESwab


Container

Dual rayon swab in Liquid Stuart Media, scored; or 1 flocked swab in ESwab transport (Note: Single rayon swab in Liquid Stuart Media is discouraged as submission does not allow for repeat testing.)

Swab in Liquid Stuart Media

Dual rayon swab in Liquid Stuart Media, scored; or 1 flocked swab in ESwab transport (Note: Single rayon swab in Liquid Stuart Media is discouraged as submission does not allow for repeat testing.)


Collection

Follow your institution's guidelines for collecting nasal swab specimens using a recommended collection and transport device, or using the following instructions: When using the dual rayon swabs, keep both swabs attached to the red cap at all times. Holding the swab cap with both swabs attached, sample each nare one at a time. Place the dual swab specimens into the transport tube containing the Liquid Stuart Medium.

When using the ESwab, collect the nasal specimen by sampling both nares one at a time with the same swab. Place the swab into the transport tube containing the Liquid Amies Transport Medium (ESwab Medium).

Moisten the swab with two drops (about 50 μL) sterile physiological saline or use it dry. Carefully insert the swab into the patient's nostril (the swab tip must be inserted up to 2.5 cm (1 inch) from the edge of the nares). Roll the swab five times. Insert the same swab into the second nostril and repeat sampling. Place the swab in its container. Label container and ship to the laboratory according to standard operating procedures.

Follow your institution's guidelines for collecting nasal swab specimens using a recommended collection and transport device, or using the following instructions: When using the dual rayon swabs, keep both swabs attached to the red cap at all times. Holding the swab cap with both swabs attached, sample each nare one at a time. Place the dual swab specimens into the transport tube containing the Liquid Stuart Medium.

When using the ESwab, collect the nasal specimen by sampling both nares one at a time with the same swab. Place the swab into the transport tube containing the Liquid Amies Transport Medium (ESwab Medium).


Storage Instructions

Refrigerated (preferred)

Store specimens at room temperature (15°C to 25°C) for 48 hours or refrigerate (2°C to 8°C) for five days.

Refrigerated (preferred)


Stability Requirements

Temperature

Period

Room temperature

24 hours (Avoid; recommend refrigerated submission)

Refrigerated

7 days

Stability provided by manufacturer or literature reference.

Temperature

Period

Room temperature

24 hours (Avoid; recommend refrigerated submission)

Refrigerated

7 days

Stability provided by manufacturer or literature reference.


Causes for Rejection

Inappropriate transport or storage conditions; name discrepancy or unlabeled specimen; expired swab transport; wire-shaft swab; swab broken at scoreline; nonflocked swab received in ESwab transport

Inappropriate transport or storage conditions; name discrepancy or unlabeled specimen; expired swab transport; wire-shaft swab

Inappropriate transport or storage conditions; name discrepancy or unlabeled specimen; expired swab transport; wire-shaft swab; swab broken at scoreline; nonflocked swab received in ESwab transport


Test Details


Use

MRSA by NAA is a qualitative in vitro diagnostic test for the direct detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA) to aid in the prevention and control of MRSA infections in health care settings.


Limitations

MRSA by NAA is not intended to diagnose MRSA infections nor to guide or monitor treatment for MRSA infections. Concomitant cultures are necessary only to recover organisms for epidemiological typing or for further susceptibility testing.


Methodology

Nucleic acid amplification (NAA)


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