Caffeine, Serum or Plasma

CPT: 80155
Updated on 11/27/2024
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Expected Turnaround Time

2 - 3 days


Related Information

FDA Cleared

FDA Cleared


Related Documents


Specimen Requirements


Specimen

Serum or plasma


Volume

1 mL


Minimum Volume

0.2 mL


Container

Red-top (no additive) tube or green-top (heparin) tube; gel-barrier tubes are not recommended

Red-top tube, lavender-top (EDTA) tube, or green-top (heparin) tube. Do not use a gel-barrier tube. The use of gel-barrier tubes is not recommended due to slow absorption of the drug by the gel. Depending on the specimen volume and storage time, the decrease in drug level due to absorption may be clinically significant.

Red-top (no additive) tube or green-top (heparin) tube; gel-barrier tubes are not recommended


Collection

Transfer separated serum or plasma to a plastic transport tube.


Storage Instructions

Submission/transport (<3 days): Room temperature. For storage beyond three days, specimen should be refrigerated or frozen.

Room temperature

Submission/transport (<3 days): Room temperature. For storage beyond three days, specimen should be refrigerated or frozen.


Stability Requirements

TemperaturePeriod
Room temperature14 days
Refrigerated14 days
Frozen14 days
Freeze/thaw cyclesStable x3

Test Details


Use

This test is used for therapeutic drug management.

Monitor caffeine concentration in newborns receiving theophylline or caffeine.

Half-life elimination: Neonates 72 to 96 hours, Children >9 months and Adults: 5 hours

Time to peak serum, oral: Within 30 minutes to 2 hours

This test is used for therapeutic drug management.


Methodology

Immunoassay (IA)

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Immunoassay (IA)


Reference Interval

8.0–20.0 µg/mL

Therapeutic: 3.0−15.0 μg/mL

• Neonatal apnea: 8−14 μg/mL

• Infant (<1 year): 8−20 μg/mL

8.0–20.0 µg/mL


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